Difference between revisions of "Part:BBa K1088016"
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<partinfo>BBa_K1088016 short</partinfo> | <partinfo>BBa_K1088016 short</partinfo> | ||
| − | This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with arabinose under glucose scarce conditions. | + | This biobrick expresses the ''Hevea brasiliensis'' rubber prenyltransferase when induced with arabinose under glucose scarce conditions. Arabinose binds the ara promoter regulator, AraC, and AraC bound arabinose activates the transcription of the ara promoter |
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| + | Using Northern blot technique it was proved that the constructs in ''E. coli'' K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details. | ||
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Revision as of 18:18, 25 September 2013
HRT2 prenyltransferase from Hevea Brasilianis (ara promoter with araC: arabinose inducible)
This biobrick expresses the Hevea brasiliensis rubber prenyltransferase when induced with arabinose under glucose scarce conditions. Arabinose binds the ara promoter regulator, AraC, and AraC bound arabinose activates the transcription of the ara promoter
Using Northern blot technique it was proved that the constructs in E. coli K-12 MG1655 doesn't express the prenyltransferase when grown without arabinose. Upon addition of arabinose to the media prenyltranferase mRNA was detected. See experince for more details.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1102 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1042
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]