Difference between revisions of "Part:BBa K1114700:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1114700 short</partinfo>
 
<partinfo>BBa_K1114700 short</partinfo>
Line 10: Line 9:
 
pI134B2C40_AE
 
pI134B2C40_AE
  
−
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114214">mCitrine_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783020">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
+
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114214">mCitrine_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783020">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_pSB1C3">pSB1C3</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
  

Revision as of 21:59, 24 September 2013

Level 1 MoClo device that constitutively expresses mCitrine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pI134B2C40_AE This is a Level 1 MoClo device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts J23104_EB, BCD2_BC, mCitrine_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.


Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits.

References