Difference between revisions of "Part:BBa K1039016"
 
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HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).
 
HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).
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=='''Sequence and Features'''==
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==Sequence and Features==
 
<partinfo>BBa_K1039016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1039016 SequenceAndFeatures</partinfo>
  
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=='''References'''==
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==References==
  
 
#Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.
 
#Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.

Latest revision as of 22:00, 17 September 2013

HPdO Helper Plasmid

HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 8168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5916
    Illegal PstI site found at 8168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2622
    Illegal XhoI site found at 8010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 8168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 8168
    Illegal AgeI site found at 5956
    Illegal AgeI site found at 6280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8173
    Illegal BsaI.rc site found at 6985



References

  1. Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.