Difference between revisions of "Part:BBa K1150043"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1150043 short</partinfo> | <partinfo>BBa_K1150043 short</partinfo> | ||
+ | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" | ||
+ | ! colspan="2" style="background:#FFBF00;"|pH1:tracrRNA pU6:DR_dummy_DR | ||
+ | |- | ||
+ | |'''Function''' | ||
+ | |two small RNAs giving rise to | ||
+ | |||
+ | specific binding of dCas9 to two VEGF loci | ||
+ | |- | ||
+ | |'''Use in''' | ||
+ | |Mammalian cells | ||
+ | |- | ||
+ | |'''RFC standard''' | ||
+ | |[https://parts.igem.org/Help:Assembly_standard_25 RFC 25] | ||
+ | |- | ||
+ | |'''Backbone''' | ||
+ | |pSB1C3<br> | ||
+ | |- | ||
+ | |'''Organism''' | ||
+ | |<i>Streptococcus pyogenes</i> | ||
+ | |- | ||
+ | |'''Source''' | ||
+ | |Feng Zhang, Addgene | ||
+ | |- | ||
+ | |'''Submitted by''' | ||
+ | |[http://2013.igem.org/Team:Freiburg Freiburg 2013] | ||
+ | |} | ||
+ | |||
+ | |||
− | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the dCas9 derivates at once. | ||
<!-- --> | <!-- --> |
Revision as of 14:39, 3 October 2013
uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3
pH1:tracrRNA pU6:DR_dummy_DR | |
---|---|
Function | two small RNAs giving rise to
specific binding of dCas9 to two VEGF loci |
Use in | Mammalian cells |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Organism | Streptococcus pyogenes |
Source | Feng Zhang, Addgene |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
By combining two single loci via the idempotent cloning strategy two loci were connected into one RNA plasmid. This enables multiple targeting of two loci with the dCas9 derivates at once.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
Illegal BglII site found at 1061 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
Illegal BsaI.rc site found at 1053