Difference between revisions of "Part:BBa K1150041"

 
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<partinfo>BBa_K1150041 short</partinfo>
 
<partinfo>BBa_K1150041 short</partinfo>
  
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|RNaimer (Bla target 3)
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|-
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|'''Function'''
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|Targeting VEGF3 with Cas9
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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===Usage and Biology===
 
===Usage and Biology===
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This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 3 .  This RNaimer is bulid of the  [https://parts.igem.org/Part:BBa_K1150034 uniCAS RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.
  
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Freiburg 2013 used this plasmid to test the efficiency of target Bla3 by activating or repressing a SEAP reporter gene.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1150041 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150041 SequenceAndFeatures</partinfo>
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Revision as of 23:12, 4 October 2013

uniCAS RNaimer to Bla Target 3


RNaimer (Bla target 3)
Function Targeting VEGF3 with Cas9
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Usage and Biology

This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 3 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 3 target.


Freiburg 2013 used this plasmid to test the efficiency of target Bla3 by activating or repressing a SEAP reporter gene.