Difference between revisions of "Part:BBa K1039017"
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HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site. | HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site. | ||
− | + | ==References== | |
− | Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991. | + | #Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991. |
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Revision as of 21:54, 17 September 2013
HPdO with deleted gVIII Helper Plasmid
HPdO with deleted gVIII Helper Plasmid contains the M13 viral genome with gVIII deleted and it is also lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011). The site from which gVIII was deleted contains a BglII restriction enzyme site.
References
- Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 7984
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5732
Illegal PstI site found at 7984 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1707
Illegal BamHI site found at 2438
Illegal XhoI site found at 7826 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 7984
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 7984
Illegal AgeI site found at 5772
Illegal AgeI site found at 6096 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7989
Illegal BsaI.rc site found at 6801