Difference between revisions of "Part:BBa K1159312"
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This parts codes for a N-terminal ''Strep''-tag-II flanked by RFC[25] N-part to allow C-terminal fusions. ''Strep''-tag II is originally designed by Prof. Arne Skerra from TU Munich.
 
This parts codes for a N-terminal ''Strep''-tag-II flanked by RFC[25] N-part to allow C-terminal fusions. ''Strep''-tag II is originally designed by Prof. Arne Skerra from TU Munich.
  
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===Usage and Biology===
 
===Usage and Biology===
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The so called N-terminal coded Strep-Tag-II flanked by RFC[25] N-part consists of the following amino acid sequence: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. It allows the protein purification after protein expression in E. coli of C-terminal fusion proteins. The iGEM Team of TU Munich 2013 used the Streptag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1159312 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1159312 SequenceAndFeatures</partinfo>

Revision as of 14:48, 23 October 2013

N-terminal Strep-tag-II in RFC[25] N-Part

This parts codes for a N-terminal Strep-tag-II flanked by RFC[25] N-part to allow C-terminal fusions. Strep-tag II is originally designed by Prof. Arne Skerra from TU Munich.


Usage and Biology

The so called N-terminal coded Strep-Tag-II flanked by RFC[25] N-part consists of the following amino acid sequence: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. It allows the protein purification after protein expression in E. coli of C-terminal fusion proteins. The iGEM Team of TU Munich 2013 used the Streptag to purify several recombinant proteins, which have been expressed in E. coli, by affinity chromatographie.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]