Difference between revisions of "Part:BBa J45993:Design"
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===References=== | ===References=== | ||
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+ | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=453110&dopt=GenBank | ||
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+ | http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684 | ||
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+ | http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938 | ||
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+ | http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.02746.x | ||
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+ | http://www.pubmedcentral.org/articlerender.fcgi?artid=178012 | ||
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+ | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-456T3CY-3&_coverDate=04%2F25%2F2002&_alid=423325879&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=7d6f4bac2242f1baa6b2b15260904531 |
Revision as of 20:45, 11 July 2006
Minimal stationary phase osmY promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
Total Length: 49 bp
Annealing Length: 21 bp
GC Content: 38.1%
Melting Temperature: 49.5 degrees C
hairpin deltaG: -2.66 kcal/mol
self dimer deltaG: -99.97 kcal/mol
Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
Total Length: 54 bp
Annealing Length: 25 bp
GC Content: 20.0%
Melting Temperature: 46.4 degrees C
hairpin deltaG: -2.86 kcal/mol
self dimer deltaG: -99.44 kcal/mol
heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
Source
This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.
References
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=453110&dopt=GenBank
http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684
http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938
http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.02746.x
http://www.pubmedcentral.org/articlerender.fcgi?artid=178012
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-456T3CY-3&_coverDate=04%2F25%2F2002&_alid=423325879&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=7d6f4bac2242f1baa6b2b15260904531