Difference between revisions of "Part:BBa K1159214"

Latest revision as of 16:30, 17 September 2013

Membrane-anchored SpyTag with secretory nLuc_SpyCatcher reporter fusion translational unit

This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory nLuc_SpyCatcher (IgKappa-SigP_nLuc_SpyCatcher) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized tag protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyCatcher Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1384
Illegal XhoI site found at 1880
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1148
Illegal AgeI site found at 1503
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 159
Illegal BsaI.rc site found at 1077
Illegal SapI.rc site found at 1581

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1159214 short</partinfo>
-This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory nLuc_SpyCatcher (IgKappa-SigP_nLuc_SpyCatcher) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyCatcher Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
+This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyTag and a secretory nLuc_SpyCatcher (IgKappa-SigP_nLuc_SpyCatcher) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized tag protein and the secretory fusion protein containing N-terminal nLuc and C-terminal SpyCatcher Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
 <!-- Add more about the biology of this part here