Difference between revisions of "Part:BBa J45992:Design"
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===Source=== | ===Source=== | ||
| − | This part was PCRed out of E. coli's genome. | + | This part was PCRed out of E. coli's genome with the primers listed in the design notes section. |
===References=== | ===References=== | ||
Revision as of 20:37, 11 July 2006
Status: 500
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Status: 500
Content-type: text/html
Software error:
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84. Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85. Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
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Design Notes
Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
Total Length: 47 bp
Annealing Length: 19 bp
GC Content: 52.6%
Melting Temperature: 53.4 degrees C
hairpin deltaG: -3.99 kcal/mol
self dimer deltaG: -98.3 kcal/mol
Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
Total Length: 54 bp
Annealing Length: 25 bp
GC Content: 20.0%
Melting Temperature: 46.4 degrees C
hairpin deltaG: -2.86 kcal/mol
self dimer deltaG: -99.44 kcal/mol
heterodimer deltaG with Forward Primer: -99.44 kcal/mol
Source
This part was PCRed out of E. coli's genome with the primers listed in the design notes section.