Difference between revisions of "Part:BBa J45992:Design"

Revision as of 20:36, 11 July 2006

Full-length stationary phase osmY promoter

Assembly Compatibility:

Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'

Total Length: 47 bp

Annealing Length: 19 bp

GC Content: 52.6%

Melting Temperature: 53.4 degrees C

hairpin deltaG: -3.99 kcal/mol

self dimer deltaG: -98.3 kcal/mol

Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'

Total Length: 54 bp

Annealing Length: 25 bp

GC Content: 20.0%

Melting Temperature: 46.4 degrees C

hairpin deltaG: -2.86 kcal/mol

self dimer deltaG: -99.44 kcal/mol

heterodimer deltaG with Forward Primer: -99.44 kcal/mol

This part was PCRed out of E. coli's genome.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
-Total Length: 49 bp
-Annealing Length: 21 bp
-GC Content: 38.1%
-Melting Temperature: 49.5 degrees C
-hairpin deltaG: -2.66 kcal/mol
-self dimer deltaG: -99.97 kcal/mol
 Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
@@ Line 50: / Line 37: @@
 heterodimer deltaG with Forward Primer: -99.44 kcal/mol
-heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
 ===Source===