Difference between revisions of "Part:BBa K1073029"

Revision as of 17:59, 24 September 2013

Inducible ampicillin resistance cassette + LuxR expression cassette

In complex with the autoinducer molecule N-3-oxo-hexanoyl-HSL, the transcription regulator LuxR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene downstream of the inducible promoter. Only LuxI, the synthase producing the autoinducer molecule N-3-oxo-hexanoyl-HSL is missing in the construct. However, N-3-oxo-hexanoyl-HSL can be taken up from the media.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1098
Illegal NheI site found at 1121
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1073029 short</partinfo>
-In complex with the inducer 3-oxo-hexanoyl-HSL, the activator LuxR binds to the Lux promoter and enables expression. This construct provides all the parts required to express the gene behind the Lux promoter. Only the inducer 3-oxo-hexanoyl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.
+In complex with the autoinducer molecule N-3-oxo-hexanoyl-HSL, the transcription regulator LuxR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene downstream of the inducible promoter. Only LuxI, the synthase producing the autoinducer molecule N-3-oxo-hexanoyl-HSL is missing in the construct. However, N-3-oxo-hexanoyl-HSL can be taken up from the media.
 <!-- Add more about the biology of this part here