Difference between revisions of "Part:BBa K1041002"
Line 6: Line 6:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
  
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K1041002 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K1041002 parameters</partinfo>
 +
<!-- -->
 
===Characterisation===
 
===Characterisation===
 
Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
 
Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
Line 27: Line 37:
 
===BLAST Analysis===
 
===BLAST Analysis===
 
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''
 
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K1041002 SequenceAndFeatures</partinfo>
 
−
 
−
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
−
<partinfo>BBa_K1041002 parameters</partinfo>
 
−
<!-- -->
 

Revision as of 11:22, 17 September 2013

AntG Promoter + RFP Coding Device

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2