Difference between revisions of "Part:BBa K1041002"
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===Usage and Biology===
 
  
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===Characterisation===
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Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
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===Sequencing===
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The biobrick was sent off to a company for sequencing.
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[[image:3a vf2 0-540.JPG|thumb|left|K1041000 sequencing data part 1]]
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[[image:3a vf2 440-860.JPG|thumb|left|K1041000 sequencing data part 2 ]]
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[[image:3a vf2 860-.JPG|thumb|left|K1041000 sequencing data part 3]]
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===BLAST Analysis===
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The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:21, 17 September 2013

AntG Promoter + RFP Coding Device

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]