Difference between revisions of "Part:BBa K1041001:Experience"

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===Team NRP-UEA_Norwich 2013===
 
===Team NRP-UEA_Norwich 2013===
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Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of [[BBa_J04450]] using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part [[Bba_K1041002]].
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Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This facilitated cloning of the biobrick [[Bba_K1041002]]
  
 
Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
 
Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Revision as of 11:14, 17 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This facilitated cloning of the biobrick Bba_K1041002

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQ5b2069eaae49fa05-partinfo-00000000-QINU UNIQ5b2069eaae49fa05-partinfo-00000001-QINU

User Reviews

UNIQ5b2069eaae49fa05-partinfo-00000002-QINU UNIQ5b2069eaae49fa05-partinfo-00000003-QINU