Difference between revisions of "Part:BBa K1060002:Design"

Revision as of 12:57, 17 September 2013

EBF synthase from Artemisia annua

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 115
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1306
Illegal BamHI site found at 615
Illegal XhoI site found at 577
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 187

Design Notes

An EcoRI site (513A>C)was removed by site directed mutagenesis

Source

Artemisia annua genomic DNA (Picaud et al. 2005)

References

 Francis, F., Martin, T., Lognay, G., and Haubruge, E. (2005). Role of (E)-b-farnesene in systematic aphid prey location by Episyrphusbalteatus larvae (Diptera: Syrphidae). European J. of Entomol., 102:431-436
 Kunert, G., Otto, S., Röse, S.R., Gershenzon, J., and Weisser, W.W. (2005). Alarm pheromones mediates production of winged dispersal morphs in aphids. Ecology letters, 8:596-603
 Picaud S, Brodelius M, Brodelius PE. (2005). Expression, purification and characterization of recombinant (E)-beta-farnesene synthase from Artemisia annua. Phytochemistry, 6(9):961-7.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1060002 short</partinfo>
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 ===References===
+  Francis, F., Martin, T., Lognay, G., and Haubruge, E. (2005). Role of (E)-b-farnesene in systematic aphid prey location by Episyrphusbalteatus larvae (Diptera: Syrphidae). European J. of Entomol., 102:431-436
+  Kunert, G., Otto, S., Röse, S.R., Gershenzon, J., and Weisser, W.W. (2005). Alarm pheromones mediates production of winged dispersal morphs in aphids. Ecology letters, 8:596-603
+  Picaud S, Brodelius M, Brodelius PE. (2005). Expression, purification and characterization of recombinant (E)-beta-farnesene synthase from ''Artemisia annua''. Phytochemistry, 6(9):961-7.