Difference between revisions of "Part:BBa K1189031:Design"
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===Design Notes=== | ===Design Notes=== | ||
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− | + | <p>This sequence was generated synthetically by <a href="http://www.genscript.com/">Genscript</a> and optimized for <i>E. coli K12</i>. As opposed to the eukaryotic Kozak seuquence on <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K782004">BBa_782004 TALE A</a> from which the TALE in this sequence was inspired, the <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> team placed a strong prokaryotic (<a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>) RBS to express it.</p> | |
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===Source=== | ===Source=== |
Revision as of 23:07, 29 September 2013
TALE-A linked to beta-lactamase with a his tag under a lacI promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1922
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2651
Illegal AgeI site found at 677
Illegal AgeI site found at 1258 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was generated synthetically by Genscript and optimized for E. coli K12. As opposed to the eukaryotic Kozak seuquence on BBa_782004 TALE A from which the TALE in this sequence was inspired, the iGEM Calgary 2013 team placed a strong prokaryotic (B0034) RBS to express it.
Source
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