Difference between revisions of "Part:BBa K1189009:Design"
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<partinfo>BBa_K1189009 short</partinfo> | <partinfo>BBa_K1189009 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | Note: The Bsa1 cut site has not been removed. | |
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− | + | TCT: Original serine - mutated to TCC: serine | |
+ | Mutagenesis Primers | ||
+ | F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’ | ||
+ | R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’ |
Revision as of 05:28, 28 September 2013
Beta-lactamase with His Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Note: The Bsa1 cut site has not been removed.
TCT: Original serine - mutated to TCC: serine
Mutagenesis Primers
F: 5’GGAGCCGGTGAGCGTGGGTCCCGCGGTATCATTGCAGC 3’
R: 5’GCTGCAATGATACCGCGGGACCCACGCTCACCGGCTCC 3’