Difference between revisions of "Part:BBa K1077006"

Latest revision as of 16:16, 29 September 2013

nat fim switch b0034 GFP OFF orientation

Design and Construction of the new natural fim switch:

Given the importance of the LRP and IHF binding sites in the switch, and the lack of these sites in the engineered fim switch we created last year, we decided to copy the natural fim switch as close as possible. The sequence of the switch varies slightly from strain to strain in E. coli. We chose to use the natural switch sequence from E. coli CFT073 because that is the strain that has the most characterization data on hbiF.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1048

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1077006 short</partinfo>
-none
+Design and Construction of the new natural fim switch:
+Given the importance of the LRP and IHF binding sites in the switch, and the lack of these sites in the engineered fim switch we created last year, we decided to copy the natural fim switch as close as possible. The sequence of the switch varies slightly from strain to strain in E. coli. We chose to use the natural switch sequence from E. coli CFT073 because that is the strain that has the most characterization data on hbiF.
 <!-- Add more about the biology of this part here