Difference between revisions of "Part:BBa K1154000"
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| − | + | Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a synthetic gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter. | |
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Revision as of 11:34, 19 September 2013
Mating pheromone-induced IGPD and constitutive LDH expression in Yeast
Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a synthetic gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 763
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]