Difference between revisions of "Part:BBa K1159108"
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By expressing this part together with its interaction partner [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] that contains the part, which interacts with PIF6, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.
 
By expressing this part together with its interaction partner [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] that contains the part, which interacts with PIF6, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:29, 16 September 2013

N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]

This parts encodes a fusion protein consisting the N-terminal half of the TEV Protease as its terminus and first 100 amino acids of PIF6 as its C-terminus. PIF6 interacts with PhyB in the presence of red light. This reaction is reversible by far-red light. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions.

By expressing this part together with its interaction partner BBa_K1159106 that contains the part, which interacts with PIF6, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316