Difference between revisions of "Help:PoPS"
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''Po''lymerase ''P''er ''S''econd: <br>
 
''Po''lymerase ''P''er ''S''econd: <br>
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Transcription initiation is defined as the number of times that an RNA Polymerase passes from the regulatory region and into the protein coding region of a BioBrick gene. PoPS are measured by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.
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Polymerase Per Second (or PoPS)  is defined as the number of times that an RNA polymerase molecule passes a specific point on DNA per unit time (e.g., from the 3' end of a promoter part into the 5' end of a mRNA). PoPS are measured indirectly by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.
  
 
This concept is related to [[Help:RiPS]], the ''Ri''bosomes ''P''er ''S''econd of a system. <br>
 
This concept is related to [[Help:RiPS]], the ''Ri''bosomes ''P''er ''S''econd of a system. <br>

Revision as of 15:51, 5 October 2006

Polymerase Per Second:
Polymerase Per Second (or PoPS) is defined as the number of times that an RNA polymerase molecule passes a specific point on DNA per unit time (e.g., from the 3' end of a promoter part into the 5' end of a mRNA). PoPS are measured indirectly by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.

This concept is related to Help:RiPS, the Ribosomes Per Second of a system.
An older term which is used synonymously with PoPs is TiPS, the Transcription initiations Per Second of a system.


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