Difference between revisions of "Part:BBa K1150003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | ... | + | This part was PCR amplified from a plasmid containing the murine G9a set-domain (A. Jeltsch, Stuttgart). Primers with overhangs containing the RFC_25 prefix and suffix were used to insert iGEM restriction sites. To eliminate illegal PstI restriction sites within the coding region of G9a two point mutations were introduced via overhangs of overlapping primers and the G9a fragments were subsequently assembled using Gibson assembly. |
Latest revision as of 22:57, 3 October 2013
G9a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was PCR amplified from a plasmid containing the murine G9a set-domain (A. Jeltsch, Stuttgart). Primers with overhangs containing the RFC_25 prefix and suffix were used to insert iGEM restriction sites. To eliminate illegal PstI restriction sites within the coding region of G9a two point mutations were introduced via overhangs of overlapping primers and the G9a fragments were subsequently assembled using Gibson assembly.
Source
...