Difference between revisions of "Part:BBa K1164008"

Revision as of 20:51, 16 September 2013

pTRELX driving yeGFP with tPGK1 terminator

This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, a lacO operator has been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 465
Illegal BamHI site found at 553
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 81
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1202

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1164008 short</partinfo>
-This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, two lacO operators have been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced.
+This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. In addition, a lacO operator has been introduced downstream of the TATA box, allowing for repression of gene expression by lacI. Upon expression from the promoter, yEGFP will be produced.