Difference between revisions of "Part:BBa K1119009:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1119009 short</partinfo>
 
<partinfo>BBa_K1119009 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Later
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There are NgoMIV & AgeI sites around MLS CDS ([[Part:BBa_K1119001|BBa_K1119001]]) and GFP reporter CDS([[Part:BBa_K648013|BBa_K648013]], which make the part incompatible with RFC25 standard.
  
  
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===Source===
 
===Source===
  
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Later
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HKUST iGEM team 2013
  
 
===References===
 
===References===

Revision as of 14:44, 26 September 2013

CMV promoter - MLS - GFP - hGH polyA tail


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 628
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1865
    Illegal BsaI.rc site found at 1367


Design Notes

There are NgoMIV & AgeI sites around MLS CDS (BBa_K1119001) and GFP reporter CDS(BBa_K648013, which make the part incompatible with RFC25 standard.


Source

HKUST iGEM team 2013

References