Difference between revisions of "Part:BBa K1119006"

Revision as of 08:59, 25 September 2013

CMV promoter

CMV (Cytomegalovirus) promoter is a constitutive mammalian promoter that we used to express glyoxylate genes in mammalian cell. Our objective in characterizing this promoter is to test if the CMV promoter drives the expression of green fluorescence protein (GFP) in mammalian HEK293FT cell line.

The partsregistry contains CMV promoter (BBa_J52034) submitted by Slovenia 2006 team and characterized by DTU-Denamrk 2011 team. In addition, the twin of this CMV promoter BBa_I712004 was submitted by Ljubljana 2007 team and characterized by Heidelberg 2009 team using BBa_K203100 pSMB_MEASURE (Promoter measurement plasmid). However, according to the user reviews, team LMU-Munich 2010 stated that this part is not a CMV promoter but rather a long version of lacI gene for prokaryotes. Although there were many uses of this promoter, our team decided to build a reliable CMV promoter for our constitutive glyoxylate shunt construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1119006 short</partinfo>
-Constitutive promoter
+CMV (Cytomegalovirus) promoter is a constitutive mammalian promoter that we used to express glyoxylate genes in mammalian cell. Our objective in characterizing this promoter is to test if the CMV promoter drives the expression of green fluorescence protein (GFP) in mammalian HEK293FT cell line.
-longer
+The partsregistry contains CMV promoter (BBa_J52034) submitted by Slovenia 2006 team and characterized by DTU-Denamrk 2011 team. In addition, the twin of this CMV promoter BBa_I712004 was submitted by Ljubljana 2007 team and characterized by Heidelberg 2009 team using BBa_K203100 pSMB_MEASURE (Promoter measurement plasmid). However, according to the user reviews, team LMU-Munich 2010 stated that this part is not a CMV promoter but rather a long version of lacI gene for prokaryotes. Although there were many uses of this promoter, our team decided to build a reliable CMV promoter for our constitutive glyoxylate shunt construct.
 <!-- Add more about the biology of this part here