Difference between revisions of "Part:BBa K1149004"

Revision as of 22:19, 2 October 2013

pelB-pueB expression

PUR degradation enzyme from Pseudomonas chlororaphis.

Characterisation

Our PueB construct was tested for growth upon induction by addition of Xylose.

PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.

Conclusion: There is no growth inhibition caused by induction in PueB.

References:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]

[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
Illegal BglII site found at 1213
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 407
Illegal AgeI site found at 752
Illegal AgeI site found at 1241
Illegal AgeI site found at 1517
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg
+<h2>Characterisation</h2>
+Our PueB construct was tested for growth upon induction by addition of Xylose.
-<p>references: </p>
+https://static.igem.org/mediawiki/2013/thumb/c/c1/PueB.png/450px-PueB.png
+PueB growth assay. E. coli (MG1655) transformed with PueB BBa_K1149004 were grown with either 0% or 2% Xylose to induce PueB expression. There was no difference between no induction and induction as a t-test gave p = 0.6040 > 0.05. Growth was at 37°C with shaking. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.
+<b>Conclusion: There is no growth inhibition caused by induction in PueB. </b>
+<h2>References: </h2>
 <p> [http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] </p>