Difference between revisions of "Part:BBa K1149004"

Line 8: Line 8:
 
<p>references: </p>
 
<p>references: </p>
  
<p> [http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/>
+
<p> [http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] </p>
<p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli] </P>  
+
<p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]</P>  
  
  

Revision as of 19:14, 15 September 2013

pelB-pueB expression

PUR degradation enzyme from

PUR_esterase_theoretical_pathway_2.jpg

references:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]

[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 1213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 407
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]