Difference between revisions of "Part:BBa K1140006"

 
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<partinfo>BBa_K1140006 short</partinfo>
 
<partinfo>BBa_K1140006 short</partinfo>
  
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This part contains a promoter regulated by TetR protein. The Transcription is regulated by a 37 oC riboswitch. The protein codified is a RFP with a LVA tag.
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Codifies for a RFP with a LVA tag. The RFP is under the regulation of a pTet promoter and a thermolabile ribosome binding site with an optimal translation temperature of 37 °C. Two transcription termination sites were also added at the 3’ end of the gene.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 01:33, 19 September 2013

pTet + 37 oC RNA thermometer + mCherry (LVA)

Codifies for a RFP with a LVA tag. The RFP is under the regulation of a pTet promoter and a thermolabile ribosome binding site with an optimal translation temperature of 37 °C. Two transcription termination sites were also added at the 3’ end of the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]