Difference between revisions of "Part:BBa K1087018:Experience"
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| + | http://2013.igem.org/Team:SCU_China/Project | ||
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| + | We ligated PO promoter with mRFP1 to create BBa_K1087018，so that we can test the basal level of PO promoter. | ||
| + | We also ligated “TetR promoter + lock3d” with phiR73 activator to create BBa_K1087007. Then, we assembly BBa_K1087007 with BBa_K1087018 to create BBa_K1087022. | ||
| + | Finally, we ligated RiboKey BBa_K145215 with BBa_K1087022 to create BBa_K1087023. | ||
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| + | We transformed BBa_K1087018, BBa_K1087022, and BBa_K1087023 into E.coli DH5α,respectively. And we use irrelevant BBa_J61046 with no fluorescence gene as negative control. The transformed cells were cultured separately in the same condition and measured | ||
| + | fluorescence value at same time. | ||
| + | |||
| + | Data showed that the basal level of PO promoter was really low, and its strength could be improved about 11 times when induced by phiR73 controlled by ribokey and lock. | ||
Latest revision as of 03:39, 28 September 2013
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1087018
User Reviews
UNIQ93bcb47c4f4f0449-partinfo-00000000-QINU UNIQ93bcb47c4f4f0449-partinfo-00000001-QINU
http://2013.igem.org/Team:SCU_China/Project
We ligated PO promoter with mRFP1 to create BBa_K1087018，so that we can test the basal level of PO promoter. We also ligated “TetR promoter + lock3d” with phiR73 activator to create BBa_K1087007. Then, we assembly BBa_K1087007 with BBa_K1087018 to create BBa_K1087022. Finally, we ligated RiboKey BBa_K145215 with BBa_K1087022 to create BBa_K1087023.
We transformed BBa_K1087018, BBa_K1087022, and BBa_K1087023 into E.coli DH5α,respectively. And we use irrelevant BBa_J61046 with no fluorescence gene as negative control. The transformed cells were cultured separately in the same condition and measured fluorescence value at same time.
Data showed that the basal level of PO promoter was really low, and its strength could be improved about 11 times when induced by phiR73 controlled by ribokey and lock.