<html><table border="1" cellspacing="0" width="100%" !important><tr><td width="100%" colspan="2"><strong><H3>Parameters for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159001">BBa_K1159001</a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</H3></strong></td></tr><tr><td width="100%" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 10</strong>: (underlined part encodes the protein)<br><span style="font-family:'Courier New', Arial;"> <u>ATGGCCGGC ... GCTACCGGT</u>TAA</span><br> <strong>ORF</strong> from 1 to 525 (excluding stop-codon)</td></tr><tr><td width="100%" colspan="2"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;"> 1 MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT<br>101 LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG<br></span></td></tr><tr><td width="100%" colspan="2"><strong>Amino acid composition:</strong><table border="0" cellspacing="0" width="100%"><tr><td width="20%"><table border="1" cellspacing="0" width="100%"><tr><td width="50%">A (Ala)</td><td width="50%">4 (2.3%)</td></tr><tr><td width="50%">R (Arg)</td><td width="50%">7 (4.0%)</td></tr><tr><td width="50%">N (Asn)</td><td width="50%">8 (4.6%)</td></tr><tr><td width="50%">D (Asp)</td><td width="50%">12 (6.9%)</td></tr></table></td><td width="20%"><table border="1" cellspacing="0" width="100%"><tr><td width="50%">C (Cys)</td><td width="50%">1 (0.6%)</td></tr><tr><td width="50%">Q (Gln)</td><td width="50%">7 (4.0%)</td></tr><tr><td width="50%">E (Glu)</td><td width="50%">8 (4.6%)</td></tr><tr><td width="50%">G (Gly)</td><td width="50%">22 (12.6%)</td></tr></table></td><td width="20%"><table border="1" cellspacing="0" width="100%"><tr><td width="50%">H (His)</td><td width="50%">4 (2.3%)</td></tr><tr><td width="50%">I (Ile)</td><td width="50%">18 (10.3%)</td></tr><tr><td width="50%">L (Leu)</td><td width="50%">16 (9.1%)</td></tr><tr><td width="50%">K (Lys)</td><td width="50%">7 (4.0%)</td></tr></table></td><td width="20%"><table border="1" cellspacing="0" width="100%"><tr><td width="50%">M (Met)</td><td width="50%">3 (1.7%)</td></tr><tr><td width="50%">F (Phe)</td><td width="50%">8 (4.6%)</td></tr><tr><td width="50%">P (Pro)</td><td width="50%">6 (3.4%)</td></tr><tr><td width="50%">S (Ser)</td><td width="50%">6 (3.4%)</td></tr></table></td><td width="20%"><table border="1" cellspacing="0" width="100%"><tr><td width="50%">T (Thr)</td><td width="50%">11 (6.3%)</td></tr><tr><td width="50%">W (Trp)</td><td width="50%">3 (1.7%)</td></tr><tr><td width="50%">Y (Tyr)</td><td width="50%">6 (3.4%)</td></tr><tr><td width="50%">V (Val)</td><td width="50%">18 (10.3%)</td></tr></table></td></tr></table></td></tr><tr><td width="40%"><strong>Amino acid counting:</strong> <table border="0" cellspacing="0" width="100%"><tr><td width=" 5%"></td><td width="55%">Total number:</td><td width="40%">175</td></tr><tr><td width=" 5%"></td><td width="55%">Positively charged (Arg+Lys):</td><td width="40%">14 (8.0%)</td></tr><tr><td width=" 5%"></td><td width="55%">Negatively charged (Asp+Glu):</td><td width="40%">20 (11.4%)</td></tr></table></td><td width="60%"><strong>Biochemical parameters:</strong><table border="0" cellspacing="0" width="100%"><tr><td width=" 5%"></td><td width="55%">Molecular mass [Da]:</td><td width="40%">19381.3</td></tr><tr><td width=" 5%"></td><td width="55%">Theoretical pI:</td><td width="40%">5.10</td></tr><tr><td width=" 5%"></td><td width="55%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td width="40%">25440 / 25503 (all Cys red/ox)</td></tr></table></td></tr><tr><td colspan="2" width="100%"><strong>Organism specific parameters</strong><table border="1" cellspacing="0" width="100%"><tr><td width="40%"><table border="0" cellspacing="0" width="100%"><tr><td><strong>Organism</strong></td></tr><tr><td><i>E. coli</i></td></tr><tr><td><i>B. subtilis</i></td></tr><tr><td><i>S. cervisiae</i></td></tr><tr><td><i>A. thaliana</i></td></tr><tr><td>Mammals</td></tr></table></td><td width="30%"><table border="0" cellspacing="0" width="100%"><tr><td><strong>Estimated half-life [h]</strong></td></tr><tr><td>>10</td></tr><tr><td>Platzhalter </td></tr><tr><td>>20</td></tr><tr><td>Platzhalter</td></tr><tr><td>30</td></tr></table></td><td width="30%"><table border="0" cellspacing="0" width="100%"><tr><td><strong>Codon usage (CAI)</strong></td></tr><tr><td>good (0.66)</td></tr><tr><td><i>B. subtilis</i></td></tr><tr><td>good (0.62)</td></tr><tr><td><i>A. thaliana</i></td></tr><tr><td>excellent (0.82)</td></tr></table></td></tr></table></td></tr><tr><td colspan="2" width="100%"><strong>Organism specific parameters</strong><table border="1" cellspacing="0" width="100%"><tr><td><strong>Organism:</strong></td><td><i>E. coli</i></td><td><i>B. subtilis</i></td><td><i>S. cervisiae</i></td><td><i>A. thaliana</i></td><td>Mammals</td></tr><tr><td><strong>Estimated half-life [h]</strong></td><td>>10</td><td>Platzhalter</td><td>>20</td><td>Platzhalter</td><td>30</td></tr><tr><td><strong>Codon usage (CAI)</strong></td><td>good (0.66)</td><td><i>B. subtilis</i></td><td>good (0.62)</td><td><i>A. thaliana</i></td><td>excellent (0.82)</td></tr></table></td></tr><tr><td colspan="2" width="100%"><strong>Sequence features:</strong></td></tr><tr><td colspan="2" width="100%"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please email us at <a href="mailto:igem@wzw.tum.de?Subject=AutoAnnotator" target="_top">igem@wzw.tum.de</a>.</td></tr></table><br></html>
Revision as of 16:33, 15 September 2013
NanoLuc Luciferase in RFC[25]
NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa).
