Difference between revisions of "Part:BBa K1033260:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1033260 short</partinfo>
 
<partinfo>BBa_K1033260 short</partinfo>
Line 16: Line 15:
  
 
===References===
 
===References===
 +
Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004

Latest revision as of 19:53, 3 October 2013

Toxin-antitoxin system from Lactobacillus plantarum


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
    Illegal SpeI site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part still contains two illegal restriction sites for EcorI and SpeI. However since it doesn't matter where in your construct you put the system it can still be assembled with XbaI and PstI.


Source

The Lactobacillus plantarum NC7 plasmid p256.

References

Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004