Difference between revisions of "Part:BBa K1064003"

 
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We use GFP generator (BBa_E0840) under the control pUV promotor (BBa_I765001) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064003). pUV promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy, while the control (without UV irradiation) doesn't show the green fluorescence [[File:PUV.jpg]]
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<partinfo>BBa_K1064003 short</partinfo>
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BBa_K1064002 constructed from pUV promoter (BBa_I765001) and GFP generator (BBa_E0840)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1064003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1064003 parameters</partinfo>
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Revision as of 03:21, 28 September 2013

We use GFP generator (BBa_E0840) under the control pUV promotor (BBa_I765001) to detect DNA damage. Our team assemble both of part becoming a new part (BBa_K1064003). pUV promotor is a promotor that regulation SOS response, this promotor will active when LexA (a protein repressor in operator) cleavage by RecA protein. When DNA was damage, Rec A protein will active by binding with ssDNA that produce when replication stop in DNA damage site. The active RecA protein will cleavage LexA (a dimer protein) that binding in operator site, this phenomenon made promotor SOS will expression the gene under its regulation. The strong RBS on BBa_E0840 allow the expression of GFP occurs after the DNA damage expossure. Here, our team test this part toward the DNA damage caused by UV irradiation and found the green fluorescence using fluorescence microscopy, while the control (without UV irradiation) doesn't show the green fluorescence PUV.jpg