Difference between revisions of "Part:BBa K1041000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of [[BBa_J04450]] using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part [[Bba_K1041002]].
+
Team NRP-UEA_Norwich 2013 improved [[BBa_J04450]] by adding an NdeI restriction site at the start of the RFP coding region of using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part [[Bba_K1041002]].
  
 
===Source===
 
===Source===

Revision as of 20:42, 29 September 2013


RFP Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Team NRP-UEA_Norwich 2013 improved BBa_J04450 by adding an NdeI restriction site at the start of the RFP coding region of using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part Bba_K1041002.

Source

BBa_J04450

References