Difference between revisions of "Part:BBa K1100013"

 
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<partinfo>BBa_K1100013 short</partinfo>
 
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<p></p>The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.
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It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
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The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.
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[[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]]
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[[File:OUTS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAOUTS1]]
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References:
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<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.
  
 
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Revision as of 15:55, 27 September 2013

R1051-OUT S1

The RNAOUTS1 is from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT.

It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%. The cI regulated promoter is a strong promoter based on the pR promoter from bacteriohage lambda. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.

Figure1. mechanism of RNA-INOUT regulatory system
Figure2. predicted structure of RNAOUTS1

References:

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]