Difference between revisions of "Part:BBa K1051802"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1051802 short</partinfo> | <partinfo>BBa_K1051802 short</partinfo> | ||
| − | This part is used to test dCas 9. | + | <p>This part is used to test dCas 9. |
| + | This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator. | ||
| + | <br/>Structure of sgRNA is like the picture below</p> | ||
| + | https://static.igem.org/mediawiki/parts/5/56/Structure_of_sgRNA.png | ||
| + | <br/>This sgRNA can help dCas9(BBa_k1051800) to target to GFP gene to inhibit the expression of GFP. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:58, 27 September 2013
sgRNA targeting to GFP gene
This part is used to test dCas 9.
This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator.
Structure of sgRNA is like the picture below
This sgRNA can help dCas9(BBa_k1051800) to target to GFP gene to inhibit the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 134
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]