Difference between revisions of "Part:BBa K1124011"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1124011 short</partinfo>
 
<partinfo>BBa_K1124011 short</partinfo>
  
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hpaBC operon (+RBS(natural))
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===Usage and Biology===
  
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This part consists of ''hpaB'' and ''hpaC'' genes, and is called ''hpaBC''
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The products of these genes, HpaB and HpaC, together form an enzyme 4-hydroxyphenylacetate 3-hydroxylase. This enzyme hydroxylates L-tyrosine to produce L-DOPA ''in vivo''.
  
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<!-- Add more about the biology of this part here
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It is known that hpaBC overexpression leads to increase of L-DOPA production in E. coli.
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===Usage and Biology===
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We designed L-DOPA sythesis device([[Part:BBa_K1124107]]) by combining this part and tyrosine synthesis device([[Part:BBa_K1124106]]).
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1124011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1124011 SequenceAndFeatures</partinfo>

Revision as of 22:43, 27 September 2013

hpaBC (+RBS(natural))

Usage and Biology

This part consists of hpaB and hpaC genes, and is called hpaBC The products of these genes, HpaB and HpaC, together form an enzyme 4-hydroxyphenylacetate 3-hydroxylase. This enzyme hydroxylates L-tyrosine to produce L-DOPA in vivo.

It is known that hpaBC overexpression leads to increase of L-DOPA production in E. coli. We designed L-DOPA sythesis device(Part:BBa_K1124107) by combining this part and tyrosine synthesis device(Part:BBa_K1124106).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1877