Difference between revisions of "Part:BBa K1124009"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1124009 short</partinfo> | <partinfo>BBa_K1124009 short</partinfo> | ||
| − | + | ===Usage and Biology=== | |
| + | '''The Effects on Hydrogen Production of HycA''' | ||
| + | Hydrogen production in ''Escherichia coli'' is performed by Formate Hydrogen Lyase complex(FHL complex), which catabolizes formate into hydrogen and carbon dioxide. FHL complex consists of proteins named HycB, HycC, HycD, HycE, HycF, HycG, and FdhF. Most of these proteins other than FdhF are coded in ''hyc'' operon, which is transcriptionally activated and inhibited by FhlA([[Part:BBa K778001]], [[Part:BBa K778002]]) and HycA, respectively. It is known that''fhlA'' overexpression increases hydrogen production in ''E. coli'', so we expects that repression of ''hycA'' expression will also lead to hydrogen production increasing. | ||
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| + | '''RNA Silencing Utilizing MicC sRNA Scaffold''' | ||
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| + | The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[BBa_K1124005]])). In this part,anti-hycA sRNA, the target-binding sequence is complementary to the first 24bp of hycA CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). | ||
| + | We expect that this part will repress ''hycA'' expression in post-transcriptional way, and that expressing this part under proper promoters may lead to hydrogen production increase. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1124009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1124009 SequenceAndFeatures</partinfo> | ||
Revision as of 18:33, 27 September 2013
anti-tyrR sRNA
Usage and Biology
The Effects on Hydrogen Production of HycA
Hydrogen production in Escherichia coli is performed by Formate Hydrogen Lyase complex(FHL complex), which catabolizes formate into hydrogen and carbon dioxide. FHL complex consists of proteins named HycB, HycC, HycD, HycE, HycF, HycG, and FdhF. Most of these proteins other than FdhF are coded in hyc operon, which is transcriptionally activated and inhibited by FhlA(Part:BBa K778001, Part:BBa K778002) and HycA, respectively. It is known thatfhlA overexpression increases hydrogen production in E. coli, so we expects that repression of hycA expression will also lead to hydrogen production increasing.
RNA Silencing Utilizing MicC sRNA Scaffold
The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold(BBa_K1124005) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold(BBa_K1124005)). In this part,anti-hycA sRNA, the target-binding sequence is complementary to the first 24bp of hycA CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). We expect that this part will repress hycA expression in post-transcriptional way, and that expressing this part under proper promoters may lead to hydrogen production increase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]