Difference between revisions of "Part:BBa K1124000:Design"

Revision as of 02:55, 12 September 2013

amilGFP (+LVA), yellow reporter protein with degradation tag

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

LVA degradation tag was added to increase protein degradation. .

13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .1

Source

PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )

References

1 Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-LVA degradation tag (sequence: AANDENYALVA) was added to increase protein degradation.   .
+LVA degradation tag was added to increase protein degradation.   .
-amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site[[1]], which may not be necessary to increase protein decay.
+amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .[[1]]
 ===Source===