Difference between revisions of "Part:BBa K1124000"
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LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
 
LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.
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<b>References</b>
 
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Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
 
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Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.
 
  
  
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<partinfo>BBa_K1124000 parameters</partinfo>
 
<partinfo>BBa_K1124000 parameters</partinfo>
 
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<b>References</b>
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Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
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Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.

Revision as of 02:40, 12 September 2013

amilGFP (+LVA), yellow reporter protein with degradation tag

Usage and Biology

The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging. It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki (currently under construction).

BBa_K1124000 is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013)(currently under construction).


amilGFP

amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. 

Excitation maximum : 503 nm
Emission maximum : 512 nm
Quantum yield : 0.67
Molar Extinction : 75200


LVA tag

LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.

Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.