Difference between revisions of "Part:BBa K1119000:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1119000 short</partinfo>
 
<partinfo>BBa_K1119000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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No
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N/A
  
  
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===Source===
 
===Source===
  
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Plasmid from Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)
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The CDS sequence of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC25 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)  
  
 
===References===
 
===References===

Revision as of 05:04, 26 September 2013

Mitochondrial Leader Sequence in RFC10 standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

The CDS sequence of MLS was cloned out from pCMV/myc/mito (Invitrogen, Carlsbard, CA) using PCR with primers that includes prefix and suffix in RFC25 standard. The original source of this CDS of MLS sequence is from that of subunit VIII of human cytochrome c oxidase. (Invitrogen, Carlsbard, CA)

References