Difference between revisions of "Part:BBa K1086000:Design"

 
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===References===
 
===References===
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Ansaldi, Mireille, et al. "The TorR High-Affinity Binding Site Plays a Key Role in Both torR Autoregulation and torCADOperon Expression in Escherichia coli." Journal of bacteriology 182.4 (2000): 961-966.

Latest revision as of 22:41, 26 September 2013


Promoter TorCAD (sensitive to TMAO)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to have 125 nucleotides for synthesis by IDT-DNA we had to add 5 randomly nucleotides in the 5' end of the sequence right above EcoRI cutting site.


Source

The source of this part is the genome of Escherichia coli (str. K-12 substr. MG1655), NCBI GI: 48994873. (Bordi et al. Mol. Micro. 2003)

References

Ansaldi, Mireille, et al. "The TorR High-Affinity Binding Site Plays a Key Role in Both torR Autoregulation and torCADOperon Expression in Escherichia coli." Journal of bacteriology 182.4 (2000): 961-966.