Difference between revisions of "Part:BBa K1104200"

 
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<partinfo>BBa_K1104200 short</partinfo>
 
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OxyR is a transcription factor found in bacteria and is activated by oxidative stress. A disulfide bond will form between 2 OxyR transcription factors to activate the transcription. In this form, OxyR induces transcription through binding to TFBS of OxyR-induced promoter. Thus, in the presence of ROS, OxyR activates transcription of the gene regulated by promoters.
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===Introduction===
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[[File: NYMU_OxyRactive.png|frame|center|'''Illustration of OxyR Activation''']]
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OxyR, a positive regulator of hydrogen peroxide-inducible genes in ''E. coli''.
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The OxyR transcription factor regulates the H<sub>2</sub>O<sub>2</sub> response of ''E. coli''. H<sub>2</sub>O<sub>2</sub> oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.
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[[File: NYMU_ORD.png|thumb|300px|center|'''Structure of the OxyR Regulatory Domain'''<br />(A)Reduced Form: Inactive<br />(B)Oxidized Form: Activated by ROS<br /> ]]
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:::::<sub>Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.</sub>
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Revision as of 15:22, 30 September 2013

OxyR

Introduction

Illustration of OxyR Activation

OxyR, a positive regulator of hydrogen peroxide-inducible genes in E. coli. The OxyR transcription factor regulates the H2O2 response of E. coli. H2O2 oxidizes OxyR Cys199 to an R-SOH that reacts with Cys208 in an intramolecular disulphide bond.

Structure of the OxyR Regulatory Domain
(A)Reduced Form: Inactive
(B)Oxidized Form: Activated by ROS
Reference: D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]