Difference between revisions of "Part:BBa K1054002"
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<partinfo>BBa_K1054002 short</partinfo> | <partinfo>BBa_K1054002 short</partinfo> | ||
| − | Holin is an essential protein for host lysis by bacteriophage. Its activity can by blocked by another protein called | + | Holin is an essential protein for host lysis by bacteriophage. This part is cloned from Bacillus subtilis subsp. subtilis str. 168. Its activity can by blocked by another protein called lrgA (BBa_K1054024) and lrgB (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid. |
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| + | <center> | ||
| + | [[File:Holin 2013zjuchina.png|600px]] | ||
| + | Growth curve of BL21 E.coli cells induced by different concentrations of IPTG | ||
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| + | </center> | ||
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Revision as of 20:36, 27 September 2013
bacteriophage PBSX holin
Holin is an essential protein for host lysis by bacteriophage. This part is cloned from Bacillus subtilis subsp. subtilis str. 168. Its activity can by blocked by another protein called lrgA (BBa_K1054024) and lrgB (BBa_K1054003). We can use this feature to prevent horizontal gene transfer of certain plasmid.
Growth curve of BL21 E.coli cells induced by different concentrations of IPTG
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]