Difference between revisions of "Part:BBa K1062000:Design"

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===Design Notes===
 
===Design Notes===
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The gRNA contains a CSY4 cut site that is essential to create the gRNA. Including in the part is a 20 nucleotide sequence that is targets specifically the RFP gene itself.
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===Sources===
 
===Sources===

Revision as of 18:27, 10 September 2013


Guide RNA (gRNA) target for RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gRNA contains a CSY4 cut site that is essential to create the gRNA. Including in the part is a 20 nucleotide sequence that is targets specifically the RFP gene itself.

Sources

The RFP gRNA was retrieved from Stanley Qi's Lab at UCSF.

References