Difference between revisions of "Part:BBa K1155003:Experience"

 
(→‎Applications of BBa_K1155003)
Line 5: Line 5:
  
 
===Applications of BBa_K1155003===
 
===Applications of BBa_K1155003===
 +
 +
We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3
 +
 +
BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)
 +
 +
What we expected :
 +
 +
+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color
 +
 +
- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color
 +
 +
What we observed :
 +
 +
+ O2 : --> purple color
 +
 +
- O2 : --> no color
 +
 +
[photo]
 +
 +
These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 22:22, 4 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1155003

We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3

BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)

What we expected :

+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color

- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color

What we observed :

+ O2 : --> purple color

- O2 : --> no color

[photo]

These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.

User Reviews

UNIQ0b5bdcb0038108c1-partinfo-00000000-QINU UNIQ0b5bdcb0038108c1-partinfo-00000001-QINU