Difference between revisions of "Part:BBa K1031421"
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<partinfo>BBa_K1031421 short</partinfo> | <partinfo>BBa_K1031421 short</partinfo> | ||
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+ | == '''Sequence and Features''' == | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1031421 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1031421 SequenceAndFeatures</partinfo> | ||
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+ | == '''Construction and data''' == | ||
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+ | For the biosensor circuit ''Ph''/J23114-HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. | ||
+ | K1031421 is composed of three elements, ''Ph'' promoter activated by HcaR, RBS B0032[https://parts.igem.org/Part:BBa_B0032] and reporter gene sfGFP ('''Fig 1a'''). Induction ratio was calculated from fluorescence intensity, showing the performance of K1031421 ('''Fig 1b''') | ||
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+ | <img src="https://static.igem.org/mediawiki/igem.org/thumb/f/f3/Peking2013_part_RBS_library_32_HcaR.png/800px-Peking2013_part_RBS_library_32_HcaR.png", width=950px; /> | ||
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+ | '''Fig 1''' Construction and tunning of reporter circuit ''Ph''-B0032-sfGFP. ('''a''') The orange arrow represents ''Ph'' promoter activated by HcaR. The green oval stands for RBS B0032[https://parts.igem.org/Part:BBa_B0032]. sfGFP coding sequence is shown with dark blue, while terminator B0015[https://parts.igem.org/Part:BBa_B0015] is in dark red. ('''b''') Horizontal axis represents biosensor circuit adopting different combination of ''Pc'' promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit ''Pc''-B0032-sfGFP in collocation with biosensor circuit J23114-HcaR. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 12:24, 25 September 2013
Ph-B0032-sfGFP-Terminator (HcaR)
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 112
Construction and data
For the biosensor circuit Ph/J23114-HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. K1031421 is composed of three elements, Ph promoter activated by HcaR, RBS B0032[1] and reporter gene sfGFP (Fig 1a). Induction ratio was calculated from fluorescence intensity, showing the performance of K1031421 (Fig 1b)
Fig 1 Construction and tunning of reporter circuit Ph-B0032-sfGFP. (a) The orange arrow represents Ph promoter activated by HcaR. The green oval stands for RBS B0032[2]. sfGFP coding sequence is shown with dark blue, while terminator B0015[3] is in dark red. (b) Horizontal axis represents biosensor circuit adopting different combination of Pc promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit Pc-B0032-sfGFP in collocation with biosensor circuit J23114-HcaR.