Difference between revisions of "Part:BBa K1223009"

Latest revision as of 13:39, 12 October 2013

pUC57 backbone (REVERSED)

pUC57 backbone - digested between BamHI and XbaI. (reverse-complement sequence)

this part is the backbone of the widely used high copy number plasmid pUC57. this plasmid is used to overexpress recombinant proteins as well as amplify a gene of interest inside E.coli. the plasmid backbone contains ampicillin resistance gene (b-lactamase) for selection of transformants.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 25
Illegal PstI site found at 2678
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 25
Illegal PstI site found at 2678
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 25
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 25
Illegal PstI site found at 2678
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 25
Illegal PstI site found at 2678
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1341
Illegal SapI.rc site found at 2423

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1223009 short</partinfo>
@@ Line 6: / Line 5: @@
 (reverse-complement sequence)
-<!-- Add more about the biology of this part here
+this part is the backbone of the widely used high copy number plasmid pUC57.
+this plasmid is used to overexpress recombinant proteins as well as amplify a gene of interest inside E.coli.
+the plasmid backbone contains ampicillin resistance gene (b-lactamase) for selection of transformants.
 ===Usage and Biology===