Difference between revisions of "Part:BBa K1031744"

Revision as of 17:06, 24 September 2013

J23114-PaaX

Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive Pc promoter J23114[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23114-PaaX

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1031744 short</partinfo>
--PaaX-TT
+<!-- -->
+== '''Construction''' ==
+In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive ''Pc'' promoter J23114[https://parts.igem.org/Part:BBa_J23114], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
+<html>
+<img src="https://static.igem.org/mediawiki/igem.org/thumb/e/e0/Peking2013_part_114-PaaX-TT.png/800px-Peking2013_part_114-PaaX-TT.png", width=600px; />
+</html>
+'''Fig 1''' Construction of biosensor circuit ''Pa''/J23114-PaaX
+== '''Sequence and Features''' ==
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1031744 SequenceAndFeatures</partinfo>