Difference between revisions of "Part:BBa K1140003:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1140003 short</partinfo>
 
<partinfo>BBa_K1140003 short</partinfo>
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Obtained by de novo synthesis.
 
Obtained by de novo synthesis.
 +
The constitutive promoter corresponds to the sequence of part BBa_J23119, the most potent member of a family of promoter variants obtained from a combinatorial library.
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Transcription factor LacI is the same one found in E. coli lac operon and the sequence employed is the same as the one from part BBa_C0012, which is the same as the one employed in the Elowitz and Leibler 2000 study as stated in the “design” section of the registry entry for part BBa_C0012, this LacI variant differs from the wild-type in that the GTG start present in nature was changed to an ATG start.
  
 
===References===
 
===References===
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 +
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8.

Revision as of 01:21, 19 September 2013

pCons + 37 oC RNA thermometer + LacI (LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 44
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

prefix: EcoRI and XbaI sufix: SpeI and PstI.


Source

Obtained by de novo synthesis. The constitutive promoter corresponds to the sequence of part BBa_J23119, the most potent member of a family of promoter variants obtained from a combinatorial library. Transcription factor LacI is the same one found in E. coli lac operon and the sequence employed is the same as the one from part BBa_C0012, which is the same as the one employed in the Elowitz and Leibler 2000 study as stated in the “design” section of the registry entry for part BBa_C0012, this LacI variant differs from the wild-type in that the GTG start present in nature was changed to an ATG start.

References

Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.